Figure 3
From: Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis
CD83 protects mice from dextran sodium sulfate (DSS)-induced colitis. (a) Decreased weight loss in Cd83Tg mice. Wild-type (WT) mice treated with 6% DSS lost ∼20% body weight, while Cd83Tg mice retained ∼89% initial body weight by day 12, n=15 animals per group. Percenatge of body weight significantly decreased in WT mice compared with Cd83Tg littermates at days 8–11 (*P<0.0001). (b) Hematoxylin and eosin staining of colon sections. Scale bar indicates 200 μm. (c) Histology scores of WT and Cd83Tg mice. WT mice had mean colitis severity score of 8.2, while Cd83Tg was significantly lower (**P=0.0094) at 5.3. Each dot represents one animal. (d) Enzyme-linked immunosorbent assay of serum cytokine levels significantly decreased in Cd83Tg mice treated with 6% DSS compared with WT littermates treated with 6% DSS. Data presented as mean±s.e.m., each dot represents average of triplicates from one animal (***P<0.0001). (e) Quantitative PCR of purified colon lamina propria dendritic cells. IL12b is significantly decreased in Cd83Tg mice treated with DSS compared with WT littermates. n=11 animals in WT+DSS group, n=10 animals in all other groups. Data presented as mean±s.d. (*P=0.0315).
