Figure 6
From: Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis
CD83 inhibits interleukin (IL)-12p40 cytokine production through cell–cell interaction. Enzyme-linked immunosorbent assays of cell culture supernatants. (a) Monocyte-derived dendritic cells co-cultured with CHO cells expressing human CD83 (gray bars) produce significantly less IL-12p40 upon stimulation with a cytokine cocktail than those co-cultured with a control CHO cell line (white bars); data presented as mean±s.d. (*P=0.0218). Results representative of data from three independent donors. (b) Mature bone marrow–derived DCs (BMDCs; gray bars) generated from Cd83 KO and wild-type (WT) littermates produce similar levels of IL-12p40 following 24 h lipopolysaccharide stimulation. (c) Co-culture of immature BMDCs with matured BMDCs from either WT or Cd83 knockout animals. Immature DCs cultured with WT mature BMDCs produced significantly less IL-12p40 than those cultured with CD83-deficient mature BMDCs, P=0.0372. Results representative of data from two independent experiments.
