Figure 8
From: Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis
CD83 affects survival to C. rodentium infection. (a, c) C. rodentium infection survival curves. (a) Survival in wild-type (WT; n=10) and Cd83Tg (n=11) animals infected with C. rodentium. Cd83Tg mice have significantly decreased survival (*P=0.0166, Log-rank test). (c) Survival in Cd83wt/wt; Cdllc-Cre (n=14) and Cd83fl/fl; Cd11c-Cre (n=11) animals infected with C. rodentium and treated with LTβR-Fc. Cd83wt/wt; Cdllc-Cre mice reach 100% mortality by day 14 after infection, while Cd83fl/fl; Cd11c-Cre animals have significantly increased survival (**P=0.0024, Log-rank test). (b, d) Il23a expression in isolated colon lamina propria dendritic cells (DCs). (b) Significant increase in Il23a in DCs from WT animals 9 days afer infection with C. rodentium compared with non-infected WT controls (*P=0.0222). No significant difference between infected and non-infected control Cd83Tg animals. DCs from infected WT animals also had significantly increased expression of Il23a compared with Cd83Tg animals infected with C. rodentium (*P=0.0424). n=5 animals in each group. (d) Significant increase in expression of Il23a in DCs isolated from Cd83fl/fl; Cd11c-Cre (conditional knockout (CKO)) animals treated with LTβR-Fc 8 days after infection compared with non-infected CKO controls (**P=0.003), while DCs from Cd83wt/wt;Cdllc-Cre (WT) animals treated with LTβR-Fc showed no significant increase in expression following infection compared with non-infected WT controls. DC expression of Il23a was significantly increased in infected CKO animals compared with WT animals infected with C. rodentium (*P=0.0175). n=6 animals per group.
