Figure 2
From: MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets
Bacterial stimulation leads to degranulation and changes in cytotoxic profile of mucosal-associated invariant T (MAIT) cells. Enriched CD8+ T cells were cocultured with THP1 cells exposed to E. coli, in the presence or absence of blocking antibodies against MR1, interleukin (IL)-12, IL-18 or isotype controls, and assayed for the expression of (a) CD107α, (b) granzyme (Gr) B, (c) perforin, (d) GrK, and (e) GrA. Representative plots gated on CD8+ T cells are shown on the left panel and cumulative data on the right panel for each marker, with bars indicating mean±s.e.m. (n=7–10). Staining control shows isotype control (conjugated with matched fluorochromes) used for gating for each marker. Note that GrA and GrK staining control is the same for each donor as both conjugated to the same flurochrome, and CD107α staining uses the unstimulated control for gating. Results are analyzed by repeated-measures one-way analysis of variance (ANOVA), with Bonferroni’s multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
