Figure 1
Increased ERK1/2 activation and IFN-γ production in Dusp6−/− CD4+ T cells. (a) Immunoblot analysis of DUSP6 expression in CD4+ T cells from WT mice upon activation of either TLR4 (100 μg LPS) or T-cell receptor (αCD3/28 antibodies). (b) Immunoblot analysis of phosphorylated levels of MAPKs in CD4+ T cells stimulated with anti-CD3/28 antibodies for the indicated time points. Data are representative of three independent experiments (a, b). (c) Cytokine levels in culture supernatants of mesenteric lymph node-derived CD4+ T cells isolated from WT and Dusp6−/− mice and stimulated with anti-CD3/28 antibodies for 24 h (n≥9 mice per group). (d) Quantitative PCR analysis of the expression of different transcription factors in CD4+ T cells from WT and Dusp6−/− mice stimulated with anti-CD3/28 antibodies (n≥5 mice per group). The mRNA fold induction in Dusp6−/− CD4+ T cells was normalized according to the mRNA expression in WT T cells. Glyceraldehyde-3-phosphate dehydrogenase was used as internal control. Error bars represent standard deviation. Data represent results from either three (a–c) or two (d) independent experiments. *P<0.05, **P<0.01. DUSP, dual-specificity phosphatase; IFN, interferon; IL, interleukin; LPS, lipopolysaccharide; NS, nonsignificant; WT, wild type.
