Figure 3
DUSP6 deficiency facilitates Th1 differentiation in vitro. (a and c) Flow cytometry analysis of intracellular cytokines in naive CD4+ T cells from WT and Dusp6−/− mice cultured in Th1 (a) or Th17 (c) polarizing conditions for 5 days, and re-stimulated with anti-CD3/28 antibodies for 6 h. (b and d) Cytokine levels measured by ELISA in supernatants of naive T cells cultured in Th1 (b) or Th17 (d) conditions and re-stimulated with anti-CD3/28 antibodies for 48 h. Data in this figure represent pooled results from four independent experiments. Error bars represent s.d. **P<0.01. DUSP, dual-specificity phosphatase; IFN, interferon; IL, interleukin; WT, wild type.
