Figure 5
DUSP6 deficiency aggravates colitis in the IL-10 model. (a) Paraffin-embedded colon samples were sectioned and stained with hematoxylin and eosin. Representative micrograph (magnification × 100) of co-housed 12-week-old Il10−/− and Il10−/−/Dusp6−/− mice maintained under specific pathogen-free conditions (n≥10 mice per group). (b) Quantitative measurement of crypt length and cellular infiltration in the different groups of mice at 12 weeks of age (n≥10 mice per group). (c) Cytokine levels in colonic explant supernatants after 24 h of culture. Represented values are normalized to milligrams of cultured colonic tissue. (d) Cytokine levels in culture supernatants of mesenteric lymph node-derived CD4+ T cells isolated from Il10−/− and Il10−/−/Dusp6−/− mice and stimulated with anti-CD3/28 antibodies for 24 h. Data represent pooled results from two independent experiments with at least six mice per group (c, d). (e) Immunoblot analysis of phosphorylated levels of ERK1/2 in splenic CD4+ T cells from both groups of mice stimulated with anti-CD3/28 antibodies for the indicated time points. Data are representative of three independent experiments. Error bars represent s.d, *P<0.05, **P<0.01. DUSP, dual-specificity phosphatase; IFN, interferon; IL, interleukin; NS, nonsignificant; TNF, tumor necrosis factor; WT, wild type.
