Figure 6
ERK inhibition ameliorates colitis in Il10−/−/Dusp6−/− mice. (a) Hematoxylin and eosin staining of Il10−/−/Dusp6−/− mice treated with the MEK1/2 inhibitor PD0325901 (PD) or vehicle five times a week for a total of 10 weeks. Representative micrographs are shown (magnification × 100; n=6 mice per group). (b) Quantitative measurement of crypt length and cellular infiltration in vehicle-treated or PD-treated mice. (c) Cytokine levels in colonic explant supernatants after 24 h of culture. Represented values are normalized to milligrams of cultured colonic tissue. Data represent pooled results from two independent experiments with at least four mice per group (b, c). Error bars represent s.d. *P<0.05, **P<0.01. DUSP, dual-specificity phosphatase; IFN, interferon; IL, interleukin; NS, nonsignificant; TNF, tumor necrosis factor; WT, wild type.
