Figure 4
From: Subversion of human intestinal mucosa innate immunity by a Crohn’s disease-associated E. coli
AIEC LF82 does not induce major disruption of human normal colonic crypts. Human normal colonic mucosa explant cultures were treated or not for 4 h with LF82 (109 bacteria per explant) or with S. Typhimurium (108 bacteria per explant). Hematoxylin-eosin (HE) standard staining and immunoperoxidase staining with the M30 antibody specific for apoptotic epithelial cells. LF82 does not alter the colonic mucosa architecture and induces only low epithelial apoptosis, mainly in the surface epithelium (brown cells, arrows), but not at the crypt base. By contrast, S. Typhimurium completely disrupts epithelial crypts including crypt base containing many M30-positive cells (asterisks).
