Figure 7
From: Subversion of human intestinal mucosa innate immunity by a Crohn’s disease-associated E. coli
AIEC LF82 does not activate the inflammasome pathway. Human normal colonic mucosa explant cultures were treated or not for 4 h with LF82 (109 bacteria per explant) or with S. Typhimurium (108 bacteria per explant), used as a positive control. (a) Caspase-1 activity detected in cell lysates, by colorimetric assay with a specific substrate (YVAD-pNA). Absorbance at 405 nm. Mean±s.e.m. of three different experiments. (b) Measurement of IL-18 secretion by ELISA in the supernatants of the 4-h explant cultures. Mean±s.e.m. of five independent experiments with at least four explant cultures per condition.
