Table 1 LF82 is able to survive in the human normal colonic mucosa-infected cells

From: Subversion of human intestinal mucosa innate immunity by a Crohn’s disease-associated E. coli

 

Sterility controls CFU/107 cells

Cell lysates plated on solid medium—CFU/107 cells

Experience 1

 Control

0

0

 LF82

0

197±84a

Experience 2

 Control

0

0

 LF82

0

442±59a

Experience 3

 Control

0

0

 LF82

0

123±33a

  1. Abbreviation: CFU, colony-forming unit. Human normal colonic mucosa explant cultures, infected or not with LF82 (109 bacteria per explant for 4 h), were then incubated with collagenase in the presence of gentamicin to kill extracellular bacteria (gentamycin protection assay), as described in Methods.
  2. aMean±s.e.m. of three cell preparations from three explants pooled in the medium containing collagenase (see Methods).
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