Figure 2
S. pneumoniae antigens recognized by serum immunoglobulin (Ig)G responses to EF3030 colonization. (a and b) Immunoblots for IgG binding to whole-cell lysates of S. pneumoniae EF3030 incubated with sera obtained on day 30 post colonization from three individual sham-colonized (a) or EF3030-colonized (b) mice. (c) Luminex assay of IgG binding to recombinant pneumococcal proteins conjugated to fluorescent beads in pooled sera obtained on day 30 post colonization from five sham-colonized (clear columns) or EF3030-colonized (black columns) mice. (d) Luminex assay of IgG binding to recombinant pneumococcal proteins conjugated to fluorescent beads in pooled bronchoalveolar lavage fluid (BALF) obtained on day 30 post colonization from five sham-colonized (clear columns) or EF3030-colonized (black columns) mice. For c and d, MFI represents binding of anti-mouse IgG-phycoerythrin secondary antibody, and error bars represent standard deviations. MFI, mean fluorescent intensity; PBS, phosphate-buffered saline.
