Figure 1

CD49d mucosal trafficking receptor expressing emTh cells at 15 dpi with B. pertussis. Mononuclear cells were collected from lungs of mice infected with B. pertussis and CD4⁺ cells were analyzed by flow cytometry and gated on CD44⁺ and CD49d⁺cells. The frequency of cells expressing CD29 and β7 was analyzed on extracellular stained cells and IL-17A- and IFNγ-expressing cells were identified by intracellular staining as detailed in materials and methods. (a) Representative histograms are shown expressing CD29, beta7 and IL-17, and IFNγ. (b) Summary of expression levels of CD29, beta7 and IL-17, and IFNγ by CD4⁺ CD44⁺ CD49d⁺ lung cells. (Histograms represent one experiment from three independent experiments. Bar graphs represent average of three independent experiments.) dpi, days post-infection; IFN, interferon; IL, interleukin; Th cells, T helper cells.

PowerPoint slide