Figure 3
Temporal frequency and trafficking of pDCs. (a) pDCs were gated among enriched lung CD11c+ cells and characterized as CD11cint, CD11b−, and mPDCA-1+. The frequency of pDCs from uninfected and B. pertussis-infected mice at 5 dpi was determined. (b) Frequency of pDCs in lungs from B. pertussis and BpΔPTX-infected mice, expressed as fold over respective cells from uninfected mice. (c) The frequency of pDCs in mediastinal lymph nodes at various times post infection is depicted in representative flow cytometry plots. (d) Bar graph representing the percentage of pDCs in mediastinal lymph nodes of mice infected with B. pertussis or BpΔPTX at various dpi. (e) GPCR expression was analyzed on lung pDCs as detailed. The frequency of pDCs expressing CCR7 and CXCR4 in the lungs of mice at 10 dpi. (Bar graphs represent averages of at least three independent experiments; **P<0.01, *P<0.05.) dpi, days post-infection; GPCR, G protein-coupled receptor; pDCs, plasmacytoid dendritic cells.
