Figure 5
Effect of IFNα on Th17 differentiation by DC in vitro. CD11c+ cells from 5 days-B. pertussis infected mice (Thy1.2+) were co-cultured for 4 days with purified CD4+ T cells from uninfected mice (Thy1.1+) with Th17-polarizing conditions as described. IFNα was added at first day of co-culture at concentrations indicated. (a) Representative flow cytometry plot and gating strategy. (b) Bar graph of the frequency of proliferating Th17 cells (IL-17+, Ki-67+). (Bar graph represents average of three independent experiments. **P<0.01, *P<0.05.) IFN, interferon; IL, interleukin; NS, not statistically significant; Th, T helper.
