Figure 1
From: Interleukin-4 and interferon-γ orchestrate an epithelial polarization in the airways
Interleukin-4 (IL-4) and interferon-γ (IFN-γ) antagonistic gene regulation. Normal human bronchial epithelial cells (NHBEs) were cultured in the presence of IL-4 or IFN-γ or IL-4 and IFN-γ for 6 h to study RNA whole transcriptome by array technology. (a) Comparison of 1,501 IL-4-upregulated entities, 3,782 IFN-γ-downregulated entities, and 7,264 IL-4/IFN-γ-regulated entities in a Venn diagram. A cut set of 48 antagonistic regulated entities was identified. The analysis is based on cultures of six genetically independent NHBEs of healthy donors. (b) A hierarchic clustering analysis of genes upregulated in comparison with untreated cells (medium) was performed, resulting in three different clusters. Labeled are six most cited genes in each cluster. (c) Comparison of 4,213 IL-4-downregulated entities, 2,412 IFN-γ-upregulated entities, and 7,264 IL-4/IFN-γ-regulated entities in a Venn diagram. A cut set of 70 antagonistic regulated entities was identified. (d) A hierarchic clustering analysis of genes upregulated by IFN-γ in comparison with untreated cells (medium) was performed, resulting in two different clusters. Labeled are the most cited genes in each cluster, except cluster 3 that only contained five genes. The color code indicates the abundance of transcripts ranging from low (blue) to high (red). Asterisks indicate two isoforms that were present in the analysis.
