Figure 2
From: Interleukin-4 and interferon-γ orchestrate an epithelial polarization in the airways
Biomarker of T helper type 1/2 (Th1/Th2) imprinted airway epithelial cells. Normal human bronchial epithelial cells (NHBEs) were cultured in the presence of interleukin-4 (IL-4) or interferon-γ (IFN-γ) or IL-4 and IFN-γ for 6 h to study RNA whole transcriptome by array technology. The analysis is based on cultures of six genetically independent NHBEs of healthy donors. (a) Comparison of 1,501 IL-4-upregulated entities, 6,219 IFN-γ-regulated entities, and 7,264 IL-4/IFN-γ-regulated entities in a Venn diagram. A cut set of 540 IL-4 only-induced entities was identified within the groups of IL-4-upregulated entities. The identified genes were listed in a ranking according to current literature using the “Genomatix” software and Gene Ontology-Terms (GO-Terms) 0007267, 0005125, 0008009, and 0005615 for identification of biomarkers resulting in a list of 22 entities. (b) Hierarchical ranking of identified IL-4-induced secreted biomarkers. Asterisks indicate two isoforms that were present in the analysis. (c) Comparison of 5,721 IL-4-regulated entities, 2,412 IFN-γ-upregulated entities, and 7,264 IL-4/IFN-γ-regulated entities in a Venn diagram. A cut set of 462 IFN-γ only induced entities was identified within the groups of IFN-γ-upregulated entities. A filter for the functions of the identified genes was listed by GO terms for identification of biomarkers resulting in a list of 22 entities. (d) Hierarchical ranking of identified IFN-γ-induced secreted biomarkers. Asterisks indicate two isoforms that were present in the analysis. The color code indicates the abundance of transcripts ranging from low (blue) to high (red).
