Figure 3
From: Interleukin-4 and interferon-γ orchestrate an epithelial polarization in the airways
Validation of in vitro gene expression changes. Normal human bronchial epithelial cells (NHBEs) were cultured in the presence of interleukin-4 (IL-4) or interferon-γ (IFN-γ) for 6 h to study RNA expression by real-time quantitative reverse transcription–PCR (qRT-PCR) confirming array results for ANO1, IL22RA1, IL24, and CCL26, expressed as log2 gene expression changes (ΔΔCt). The analysis is based on cultures of six genetically independent NHBEs of healthy donors. Data have been log2 transformed to get a normal distribution, and the Shapiro test for normal distribution was performed. The genes are normally distributed on a 1% significance threshold.
