Figure 4
From: Interleukin-4 and interferon-γ orchestrate an epithelial polarization in the airways
Antagonistic transcriptional regulation. Normal human bronchial epithelial cells (NHBEs) were cultured in the presence of interleukin-4 (IL-4) or interferon-γ (IFN-γ) or IL-4 and IFN-γ for 6 h to study RNA whole transcriptome by array technology. The analysis is based on cultures of six genetically independent NHBEs of healthy donors. (a) Comparison of 1,501 IL-4-upregulated entities, 3,782 IFN-γ-downregulated entities, and 7,264 IL-4/IFN-γ-regulated entities in a Venn diagram. A cut set of 768 entities was identified within the groups of IL-4-upregulated and IL-4/IFN-γ-regulated entities. The identified genes were listed in a ranking according to current literature using the "Genomatix" software and Gene Ontology-Terms (GO-Terms) 0003676 and 0044212 for transcription factors resulting in a list of 80 entities. (b) Hierarchical ranking of identified IL-4-induced transcription factors, containing IL-4-master transcription factor GATA3. (c) Comparison of 4,213 IL-4-downregulated entities, 2,412 IFN-γ-upregulated entities, and 7,264 IL-4/IFN-γ-regulated entities in a Venn diagram. A cut set of 1,779 entities was identified within the groups of IL-4-upregulated and IL-4/IFN-γ-regulated entities. (d) Hierarchical ranking of identified IFN-γ-induced transcription factors, containing IFN-γ-master transcription factor TBX21. The color code indicates the abundance of transcripts ranging from low (blue) to high (red).
