Table 1 Surface antigens used to characterize mouse lung cell subsets by flow cytometry

From: A pathogenic role for tumor necrosis factor-related apoptosis-inducing ligand in chronic obstructive pulmonary disease

Cell subset

Cell surface antigens

CD11b+ monocyte

CD45+ F4/80+ CD11c− CD11b+ TRAIL+/−

Neutrophil

CD45+ F4/80− CD11c− CD11bhi GR-1hi TRAIL+/−

γδT cell

CD45+ CD3+ CD4− CD8− γδTCR+ TRAIL+/−

NKT cell

CD45+ CD3+ αGalCer tetramer+ TRAIL+/−

CD4+ T cell

CD45+ CD3+ CD4+ CD8− γδTCR− TRAIL+/−

CD8+ T cell

CD45+ CD3+ CD4− CD8+ γδTCR− TRAIL+/−

mDC

CD45+ F4/80− CD11c+ CD11b+ PDCA− TRAIL+/−

Alveolar macrophage

CD45+ F4/80+ CD11c+ CD11b− TRAIL+/−

pDC

CD45+ F4/80− CD11clo CD11b− PDCA+ TRAIL+/−

  1. Abbreviations: γδT, gamma delta T cells; NKT cell, natural killer T cell; mDC, myeloid dendritic cell; pDC, plasmacytoid dendritic cell.
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