Figure 2
From: Intestinal FoxO signaling is required to survive oral infection in Drosophila
Oral infection with Serratia marcescens Db11 activates different immune-dependent signaling pathways in enterocytes. Different reporter constructs targeted to enterocytes (ECs) were used to elucidate the signaling pathways involved in the epithelial immune response to an oral infection with S. marcescens Db11. NP1-Gal4>UAS-PLCß-mRFP (a), which express a RFP-tagged fusion with PLCß in ECs only, showed cytoplasmic localization under control conditions (top row), whereas infection-induced translocation to the membrane (bottom row). Infection was able to activate green fluorescent protein (GFP) expression in CaLexA flies, indicative for prolonged Ca2+ activation in enterocytes. NP1-Gal4>UAS-Relish-YFP drives expression of a YFP-tagged Relish protein to ECs (b). Nuclear translocation was not observed under control conditions (top row), or following infection with S. marcescens (bottom row). NP1-Gal4>UAS-dFoxO-GFP targets a GFP-tagged dFoxO protein to ECs (c). Under control conditions (top row), the signal was found in the cytoplasm, while infection with S. marcescens induced its nuclear translocation (bottom row). Bar=20 μm.
