Figure 4
From: Intestinal FoxO signaling is required to survive oral infection in Drosophila
Effect of oral infection on lifespan and bacterial load in IMD- and dFoxO-deficient animals. Males (a and c) and females (b and d) of the different genotypes w1118 (control, black), rel38 (white), foxo21/21 (grey) were fed with Db11 (OD600>50; c and d) or diluent only (5% sucrose; a and b) throughout their adult life, and the number of survivors was quantified every second day. n=100 individuals each. Statistical analysis was performed with the Mantel–Cox log-rank test (Supplementary Table 2). For the bacterial load assays, adult animals of males (e) and females (f) were fed with Serratia DB11 (OD1 in 5% sucrose) for 2 h that was supplemented with blue food dye to allow quantification of the ingested bacteria (starting points) and then placed on normal medium. Labeling of genotypes was as in a–d and the mean values±s.e.m. (four biological replicates) are displayed. ** P<0.01, t-test.
