Figure 2
From: IL-36α expression is elevated in ulcerative colitis and promotes colonic inflammation
IL-36-receptor-deficient mice suffer from less severe disease in an acute model of DSS-induced colitis. (a) Levels of gene expression of IL-36 family members in colons of WT mice were measured after 1 and 6 days treatment with 2% DSS by qPCR. WT ctrl and il36r−/− mice (six per group) were administered 2% DSS ad libitum in drinking water for 6 days and the development of colitis monitored by (b) disease activity index including fecal occult blood and stool consistency as well as (c) weight loss. After 6 days, the experiment was terminated and (d,e) colon length, (f,g) histology of colon tissues and (h) levels of MPO activity were examined to determine levels of inflammation/tissue destruction. Data representative of four separate DSS experiments (5–7 mice per group) with similar results. Statistical analysis performed by unpaired student t-test, *P≤0.05, **P≤0.01, and ***P≤0.001. DSS, dextran sodium sulfate; MPO, myeloperoxidase; qPCR, quantitative PCR; WT, wild-type.
