Figure 5
From: IL-36α expression is elevated in ulcerative colitis and promotes colonic inflammation
IL-36-deficient mice display altered mucosal Th subset responses in C. rodentium-induced colitis. (a) Relative gene expression heat map from the distal colon mRNA from (il36+/−) littermate ctrl and il36r−/− mice analyzed on day 7 and 14 p.i. for the main markers representative of the Th17 (Il17a, Il23, Il6, IL1b, and Rorgt), Th1 (Ifng, Il12b, and Tbet), and Treg (Foxp3 and Il10) responses. Color scales show higher relative expression in red and lower relative expression in blue. Each box represents specific gene expression values for individual mice (b,c) Bars represent the mean± s.e.m. relative expression of each gene from the colons of 5–7 individual mice at (b) day 7 and (c) day 14 post infection, using the values from uninfected ctrl and il36r−/− mice as their respective baseline. Significance was determined by Mann–Whitney t-test *P<0.05 and **P<0.01. Fluorescence activated cell sorting (FACS) analysis of (d) CD4+IFNγ+ and (e) CD4+IL-17A+ cells in the mesenteric lymph nodes of uninfected and infected ctrl and il36r−/− mice at day 7 p.i. Each symbol represents values for an individual mouse with five mice per group. (f) Representative FACS showing values from individual mice in e. Data expressed as mean±s.e.m. with significance determined by unpaired student’s t-test, *P≤0.05.
