Figure 1
Complement activation in azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis-associated cancer. (a) Schematic overview of colitis-associated cancer (CAC) regimen. (b, c) At the indicated time points after initiation of CAC induction, colons were homogenated and supernatants were prepared for detection of (b) C3a and (c) C5a protein levels. Each group consisted of 6–8 mice. (d) C3 deposition in the colons at the indicated time points after CAC was detected by immunohistochemistry. Scale bar=100 μm. The data were pooled from two independent experiments. Each group consists of 6 to 8 mice. *P<0.05, **P<0.01. D, day.
