Figure 3
Interleukin-22 (IL-22)/type I interferon (IFN) synergy increases signal transducer and activator of transcription factor (STAT)1 but not STAT3 activation in intestinal epithelial cells (IEC). (a,b) Intestinal epithelial HT29 cells were cultured in the presence of both IL-22 and IFN-α added at the same time. Phosphorylated STAT (P-STAT) analyses were performed by flow cytometry. (a) Data represent cells positive for the indicated P-STAT molecule after 15 min of culture in the presence of IL-22 alone (green), IFN-α alone (blue) or IL-22 and IFN-α (red) as compared as non-stimulated condition (grey). Data represent one of three independent experiments. (b) Data represent mean±s.e.m. of the ratio of the mean of fluorescence intensity (MFI) of stimulated cells with the unstimulated condition at different time points (n=3). (c–e) Where indicated, intestinal epithelial HT29 cells were cultured for 15 h in the presence of IFN-α. (c,d) Where indicated, IL-22 was added to the medium for 30 min before P-STAT staining. (e) Where indicated, IL-22 was added to the medium for 24 h and culture supernatant hCXCL10 analyses were performed by enzyme-linked immunosorbent assay. *P<0.05, ***P<0.001.
