Figure 6
Expression of CXC-chemokine receptor 3 (CXCR3) is increased in Th cells of mice experiencing intestinal graft-versus-host disease (GVHD). (a–d) Lethally irradiated wild-typr (WT) or type I IFN receptor deficient (IFNAR−/−) 129sv recipients were transplanted with 5 × 106 B6 WT or IL-22 deficient (IL-22−/−) bone marrow (BM) only or with 10 × 106 B6.WT splenocytes or 10 × 106 B6.IL-22−/− splenocytes (n=5 per group and five independent experiments). At day 20 post transplantation, mesenteric lymph nodes (MLNs) were collected. Extracellular expression of CXCR3 was analyzed CD4+ T cells by flow cytometry. (a) Expression of CXCR3 on gated CD4+ T cells is shown for a representative sample. (b) Mean±s.e.m. of the percentage of CD4+ T cells expressing CXCR3 in the MLNs is shown. (c,d) At day 20 post transplantation, colons were excised for quantitative reverse transcriptase–PCR analysis for each indicated gene. Data are combined from three independent experiments (n=12).
