Figure 7
Interleukin-22 (IL-22) in donor cells and intact type I interferon (IFN) signaling pathway in recipient mice are required for increased inflammatory mediator production. (a–c) Lethally irradiated wild-type (WT) or type I IFN receptor deficient (IFNAR−/−) 129sv recipients were transplanted with 5 × 106 B6 WT or IL-22-deficient (IL-22−/−) BM only or with 10 × 106 B6.WT splenocytes or 10 × 106 B6.IL-22−/− splenocytes (n=5 per group). Spleen and mesenteric lymph nodes (MLNs) were collected at day 20 post allogeneic hematopoietic cell transplantation (allo-HCT). Splenocytes and MLN cells were activated for 6 h with phorbol-12-myristate-13-acetate (PMA), ionomycin, and brefeldin A. Intracellular IFN-γ and IL-17 in CD4+ T cells were analyzed. (a) Expression of IL-17 and IFN-γ on gated CD4+ T cells is shown for a representative sample. (b,c) Mean±s.e.m. of the percentage of CD4+ T cells secreting IFN-γ (IFN-γ+IL-17+ and IFN-γ+IL-17−) in the MLN and spleen is shown. Data are combined from three independent experiments (n=15).
