Figure 3

MHCII⁺ monocytes and pathogenic T cells are reduced in Helicobacter hepaticus (Hh)-infected and anti-IL-10R-treated CD11c^IL-23- mice. CD11c^IL-23+ and CD11c^IL-23- mice were infected with Hh combined with anti-IL-10R monoclonal antibody (mAb) treatment and analyzed after 3 weeks. (a) Representative fluorescence-activated cell sorting (FACS) plots, frequencies among CD45⁺ leukocytes, and absolute numbers of the indicated myeloid subsets in the colonic lamina propria. (b) Frequencies of CD4⁺ T cells among CD45⁺ leukocytes. (c) Representative FACS plots and frequencies of IFNγ⁺, IFNγ⁺ IL-17A⁺, and IL-17A⁺ cells among CD4⁺ T cells upon restimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Data points represent individual mice, bars indicate medians. Data are representative of two independent experiments. *P<0.05, **P<0.01 as determined by Mann–Whitney U-test. IFNγ, interferon-γ; IL, interleukin; MHCII, major histocompatibility complex class II; TCR, T cell receptor.

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