Figure 5

Interleukin-23 (IL-23) production during colitis is restricted to CX₃CR1-expressing CD64⁺ subsets. CX₃CR1^GFP/+ mice were infected with Helicobacter hepaticus (Hh) combined with anti-IL-10R monoclonal antibody (mAb) treatment and analyzed 2 weeks after infection alongside uninfected controls (Ctrl). Colonic lamina propria cells were sorted by flow cytometry and Il23a mRNA expression was analyzed by quantitative PCR (qPCR). (a) Il23a mRNA expression and representative fluorescence-activated cell sorting (FACS) plots of the following sorted populations: (1) MHCII⁺ monocytes (Mono), (2) Ly6C^lo CX₃CR1^int macrophages/DCs, (3) CX₃CR1^hi macrophages, and (4) CD103⁺ DCs. (b) Il23a mRNA expression and representative FACS plots of the following sorted populations: (1) CX₃CR1^int MHCII⁺ CD64^- and (2) CX₃CR1^int MHCII⁺ CD64⁺ cells. Bar charts represent the mean+s.e.m. of 10–15 pooled mice, sorted in two biological replicates. Data are representative of three independent experiments. **P<0.01, ***P<0.001, as determined by one-way analysis of variance (ANOVA) with Bonferroni’s post-test. DC, dendritic cell; Macs, macrophages; MHCII, major histocompatibility complex class II; Mono, monocyte; NS, not significant.

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