Figure 7
Macrophages share functional redundancy with MHCII+ monocytes for interleukin-23 (IL-23) production. CX3CR1GFP/+ mice were treated with a blocking anti-CSF-1R monoclonal antibody (mAb) or isotype (Iso) control and analyzed (a–e) 4 days or (f, g) 2 weeks after Helicobacter hepaticus (Hh) and anti-IL-10R mAb treatment. (a) Representative fluorescence-activated cell sorting (FACS) plots and (b) frequencies among CD45+ leukocytes of the indicated myeloid subsets, as defined in Figure 1c. (c) Total number of colonic lamina propria cells per mouse. (d) Frequencies of neutrophils among CD45+ cells. (e) Quantitative PCR (qPCR) analysis of Il23a mRNA expression by total colonic lamina propria cells analyzed at day 4. (f) Total number of colonic lamina propria cells per mouse 2 weeks after Hh and anti-IL-10R mAb treatment. (g) Colonic histopathology scores. Data points represent individual mice, bars indicate medians. Data are pooled from two experiments and representative of three independent experiments. *P<0.05, **P<0.01, as determined by Mann–Whitney U-test. CSF, colony-stimulating factor; DC, dendritic cell; Macs, macrophages; MHCII, major histocompatibility complex class II; NS, not significant; Uninf, uninfected.
