Figure 6
p21 is involved in low dM permissivity to HIV-1. (a) Representative western blot of p21 expression by freshly purified dM (day 0) and by US dM at day 7 of culture (left panel). The protein band intensity was quantified and normalized to the actin band. Ratio (p21/actin) was then compared between dM at day 0 and day 7 (right panel) (n=6). (b) Representative western blot of p21 expression by IFN-γ-stimulated and US dM on day 7 of culture (left panel). Ratio (p21/actin) was then compared between the 7-day IFN-γ-stimulated and US (right panel) (n=20). (c) Fold changes of IRF5 and p21 expression were calculated by comparing the normalized value of IRF5 and p21 from the IFN-γ-treated samples to the normalized value of IRF5 and p21 from the dM (US) control. Correlation between fold changes in IRF5 and p21 expression on day 7 of IFN-γ treatment compared with time-matched US dM (n=19). Spearman's R value is shown. (d) Representative western blot of p21 expression in 7-day IFN-γ-stimulated and US dM transfected with p21-specific siRNA or with an irrelevant control siRNA (Ctrl) (n=9). (e) Number of late reverse transcription products (U5GAG) and (f) integrated forms in dM transfected with p21-specific or control siRNA (n=9). Mean±s.e.m. of n independent donors. dM, decidual macrophages; IFN, interferon; siRNA, short interfering RNA; US, unstimulated.
