Figure 9
TLR7/8 triggering in decidual explants induces M1 polarization of dM and restricts HIV-1 replication. Decidual explants were stimulated with R848 or left US. (a–d) Cytokine and β-chemokine concentrations in explant supernatants after 3 days of culture. (e–g) Marker expression on CD45+ CD14+ dM after explant digestion on day 7 of culture, as measured by flow cytometry of total decidual cells. The % change in MFI induced by R848 and IFN-γ was determined by comparison with time-matched US explants. (h) Explants were stimulated with R848 or left untreated prior to challenge with HIV-1BaL. HIV-1 p24 Ag was measured by ELISA in explant supernatants. Mean±s.e.m. of 7 independent donors. #=statistical comparison of MFI values between R848-stimulated vs. US dM. dM, decidual macrophages; MFI, mean fluorescent intensity; TLR, Toll-like receptor; US, unstimulated.
