Table 1 Gating strategy for flow cytometry analysis

From: Neutrophilic NLRP3 inflammasome-dependent IL-1β secretion regulates the γδT17 cell response in respiratory bacterial infections

Populations

Markers

Lymphoid compartment

All CD45+

γδT cells

CD3+ TCRδ+

ILCs

Lin− CD90.2+ CD127+

NKT cells

PBS57/CD1d tetramer+ TCRβ+

NK cells

CD3− NK1.1+

conventional T cells

PBS57/CD1d tetramer− TCRβ+

Myeloid compartment

All CD45+

Neutrophils

CD11b+ Ly6G+

AMs

CD11b+ Siglec F+

Interstitial macrophages

CD11b+ F4/80+

conventional DCs

CD11c+ MHCII+ CD64−

  1. Abbreviations: AM, alveolar macrophage; DC, dendritic cell; NKT, natural killer T.
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