Figure 4
Detection of cervix-derived HSV-2-reactive CD8+ T cells using autologous HSV-2-infected DCs. The cells isolated from cervical biopsy samples were co-cultured with autologous mock-infected or HSV-2-infected DCs and tested ex vivo for the expression of IFN-γ and CD103. (a) Representative dotplots for the expression of IFN-γ and CD103 by CD8+ T cells isolated from a cervical biopsy in response to mock DCs (bottom graphs) or HSV-2 DCs (top graphs). T cells were isolated from a cervical biopsy from a representative HSV-2-infected participant and co-cultured with autologous mock DCs or HSV-2 DCs and the resultant CD8+ T cells were analyzed by ICS and flow cytometry for the expression of IFN-γ and CD103. (b) Net % HSV-2-reactive CD8+ T cells (CD8+/IFN-γ+) was measured in biopsy samples from five HSV-2-infected subjects; the bar depicts the median of all cervical biopsy samples. (c) Expression of CD103 on HSV-2-reactive CD8+ T cells derived from cervical biopsies. The percentage contribution of IFN-γ+ (HSV-2-reactive) cells to the total CD103(+) and CD103(−) CD8+ T-cell subsets from five participants is displayed. Bars represent the medians.
