Figure 3
Effect of interleukin-36γ (IL-36γ) on lung bacterial clearance and dissemination. (a and b) Mice were passively immunized with either a control immunoglobulin G (IgG) or an anti-IL-36γ IgG and infected with intrathecal (i.t.) Streptococcus pneumoniae (Sp). Lungs and spleen were harvested at the specified time points. (a) Lung colony-forming units (CFUs) were assessed by serial dilution (*P<0.01 as compared with control IgG-treated mice by two-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test, n=4 mice per group, representative of two experiments). (b) Spleen CFUs were assessed by serial dilution at 48 h (*P<0.001 compared with control IgG-treated mice by two-tailed Student’s t-test, n=4 mice per group, representative of two experiments). (c and d) Wild-type (WT) and IL-36γ−/− were infected with intrathecal (i.t.) Sp, and lungs and spleen were harvested at 48 h after infection. (c) Lung CFUs were assessed by serial dilution at 48 h (*P<0.05 compared with WT by two-tailed Student’s t-test, n=4 mice per group, representative of three experiments). (d) Spleen CFUs were assessed by serial dilution at 48 h (*P<0.001 compared with WT by two-tailed Student’s t-test, n=4 mice per group, representative of three experiments).
