Figure 5
Effect of interleukin-36γ (IL-36γ) on inflammatory cell recruitment: (a) Wild-type (WT) mice were passively immunized with either a control immunoglobulin G (IgG) or anti-IL-36γ IgG and infected with intrathecal (i.t.) Streptococcus pneumoniae (Sp). Total bronchoalveolar lavage (BAL) leukocytes were quantified, and macrophage and neutrophil counts were assessed by manual differential (no significant differences between groups at the same time point by one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test, n=5 mice per group, representative of two experiments). (b) WT and IL-36γ−/− were infected with i.t. Sp. Total leukocytes, macrophages, and neutrophils were assessed as above (no significant differences between groups at the same time point by one-way ANOVA with Sidak’s multiple comparisons test, n=4–6 mice per group, representative of three experiments). (c) WT and IL-36γ−/− were infected with i.t. Sp. Lungs were homogenized at the specified time points, and total leukocytes, macrophages, and neutrophils were assessed by manual differential (no significant differences between groups at the same time point by one-way ANOVA with Sidak’s multiple comparisons test, n=3 mice per group per time point). Unstim, unstimulated.
