Figure 7
Effect of interleukin-36γ (IL-36γ) on dendritic cells expression of type-1 and IL-17 cytokines. (a) Bone marrow-derived dendritic cells (BMDCs) isolated from wild-type (WT), MyD88−/−, IL-36R−/−, and TLR4−/− mice were stimulated with increasing concentrations of recombinant IL-36γ. Cytokine secretion was measured by ELISA (enzyme-linked immunosorbent assay) at 6 h (*P<0.001 as compared with WT control by two-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test, n=4 per group, representative of two experiments). (b) BMDCs were isolated from WT mice were cocultured with either no microparticles (MPs) (WT), MP isolated from WT pulmonary macrophages (PMs) treated with a control (IgG) or an anti-IL-36γ IgG (αIL-36γ IgG), or MP isolated from IL-36γ−/− PM. Cytokines were measured by ELISA at 6 and 24 h (*P<0.01 compared with WT at the same time point by two-way ANOVA with Dunnett’s multiple comparisons test, n=4–5 per group, representative of two experiments). TLR, Toll-like receptor.
