Figure 8
Effect of interleukin-36γ (IL-36γ)-containing microparticles on lung bacterial clearance. (a) Wild-type (WT) or IL-36γ−/− were treated with either vehicle or microparticles (MPs) derived from pulmonary macrophages (PMs) of either WT or IL-36γ−/− mice as specified, and then infected with intrathecal (i.t.) Streptococcus pneumoniae (Sp). Lung colony-forming units (CFUs) were assessed by serial dilution at 48 h after infection (*P<0.001 compared with WT/vehicle by one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test, n=5 mice per group, representative of two experiments). (b) WT or IL-36γ−/− were treated with MP derived from PM of either WT or IL-36γ−/− mice as specified, and then infected with i.t. Sp. IL-12p40, IL-23p19, and IFNγ were assessed by serial dilution at 48 h after infection (*P<0.001 compared with WT/vehicle by one-way ANOVA with Dunnett’s multiple comparisons test, n=5 mice per group, representative of two experiments).
