Figure 1
From: Immune correlates of protection against HIV infection and how to elicit them
Messenger RNA (mRNA) expression of genes that are part of the type I interferon (IFN) signal transduction pathway in peripheral blood mononuclear cells (PBMC) that were cultured in medium containing two different thiazolides: tizoxanide (TIZ) or RM-4848, and were or were not in vitro infected with HIV (the R5 tropic HIV-1BaL strain was used in the experiments shown herein). mRNA expression was assessed by real-time quantitative polymerase chain reaction, calculated relative to five housekeeping genes and shown as fold-change expression from the untreated sample. Gene expression (n-fold) is shown as a color scale from green to red (−2 to +9) (MEV multiple experiment viewer software); only genes that are upregulated are shown. (a) PBMC cultured in medium containing TIZ (10 μg/ml) that were either uninfected or HIV1-infected. Results at day 7 post infection are shown. (b) PBMC cultured in medium containing RM-4848 (10 μg/ml) that were either uninfected or HIV1-infected. Results at day 7 post infection are shown.
