Figure 2
Dysbiosis index based on differentially distributed taxa. (a) A cladogram illustrating phylogenetic relationship between taxa, the center dot representing the kingdom Bacteria, the first circle representing Phylum, then the Class, Order, Family, and Genus levels. Taxa that are increased in common variable immunodeficiency (CVID) compared with controls are in red and taxa that are reduced in CVID compared with controls are in green. Named taxa are significant according to both univariate and multivariate statistics (Table 1) and are marked as small letters in the cladogram referring to corresponding taxa names in the legend at the right side of the figure. The vertical lines on the left side of the legend define taxa representing different levels of the same branch. The phylogenetic tree and coloring were made using LEfSe.24 (b) CVID-specific microbiota dysbiosis index (calculated from the abundances of the ten clades shown in (a)) applied in CVID, inflammatory bowel disease (IBD) and controls showed in median, quartiles, and range. The comparison is made by using Mann–Whitney U test; significant P-value is marked as ***P<0.001, **P<0.01,*P<0.05. (c) Dysbiosis index correlates with alpha diversity (Chao1), Rho refers to Spearman's rank correlation coefficient.
