Figure 5
From: IL-27 suppresses type 2 immune responses in vivo via direct effects on group 2 innate lymphoid cells
IL-27 as a negative regulator of ILC2-mediated anti-helminthic immunity. C57BL/6 and Ebi3−/− mice were infected with 500 L3 N. brasiliensis larvae and analyzed 7 d.p.i. ILC2 frequencies in lungs (a) and tLNs (b) were analyzed by flow cytometry. Plots were pregated on lin- cells. Graphs on the right depict the quantification of absolute ILC2 numbers per organ. IL-5 and IL-13 concentrations in sera (c) or supernatants of PMA/Ionomycin stimulated lung leukocytes (d) were determined by specific ELISAs. (e) Small intestinal worm burdens were enumerated. (f) Small intestinal tissue sections were stained with PAS and goblet cells per villus were quantified. (g, h) mRNAs of indicated genes were quantified in total RNA of small intestinal tissue lysates by qRT-PCR. Data represent 3 independent experiments with 4-5 mice per group. Error bars indicate s.e.m.
