Figure 2
Caspase-8 deficiency markedly attenuates Th2 immune response in the allergic asthma model. (a) T cells from OVA-treated WT, Rip3−/−, and Rip3−/−Casp8−/−mice were analyzed for IL-4, IFN-γ, and IL-17 expression by intracellular cytokine staining. (b) Percentage of T cells producing IL-4, IFN-γ, and IL-17 relative to total CD4+ or CD8+ T cells in a. (c) The total number of T cells producing IL-4, IFN-γ, and IL-17 in a. (d) Reverse transcription PCR analysis of Il4, Il5, Il13, and Ifng in OVA-treated WT, Rip3−/−, and Rip3−/−Casp8−/− mice. (e) ELISA analysis of IL-4, IL-5, IL-13, and IFN-γ in OVA-treated WT, Rip3−/−, and Rip3−/−Casp8−/− mice. Each symbol indicates an individual mouse and mean±s.e.m. values are shown. Results are representative of three independent experiments. **P<0.01; ***P<0.001; ****P<0.0001; IFN-γ, interferon-γ; IL, interleukin; NS, not significant; OVA, ovalbumin; WT, wild type.
