Figure 3
Contribution of hematopoietic and radioresistant cells to the protected phenotype of Rip3−/−Casp8−/− mice in response to OVA administration. (a and b) Bone marrow chimeric mice (> indicates bone marrow donor cells transferred to recipient mice) were sensitized with OVA/alum and challenged with OVA. Pulmonary immune cell infiltration (a) and CD4+ T cells producing IL-4 (b) were analyzed by fluorescence-activated cell sorting 24 h after the last OVA challenge. (c and d) Dendritic cells sorted from Rip3−/− and Rip3−/−Casp8−/− mice and co-cultured with OVA-primed CD4+ T cells in the presence or absence of the OVA peptide (1 μg ml−l) in vitro for 5 days. The proliferated T cells were stimulated with phorbol myristate acetate and ionomycin, and analyzed for cytokine production. The percentage of total CD4+ T cells (c) and total CD4+ T cells producing IL-4, IFN-γ, and IL-17 (d) are shown. Results are pooled from two independent experiments. *P<0.05; **P<0.01; ***P<0.001; ****P< 0.0001; IFN-γ, interferon-γ; IL, interleukin; NS, not significant; OVA, ovalbumin.
