Figure 5
The activity of Rip3−/−Casp8−/− dendritic cells for promoting Th2 cell differentiation is restored by exogenous application of IL-1α or IL-β. Dendritic cells sorted from naive Rip3−/− and Rip3−/−Casp8−/− mice and co-cultured with OVA-primed CD4+ T cells with or without the exogenous application of IL-1α (40 ng ml−l), IL-1β (40 ng ml−1), or both in the presence of the OVA peptide (1 μg ml−1) for 5 days. The proliferated T cells were stimulated with phorbol myristate acetate and ionomycin, and analyzed by fluorescence-activated cell sorting for cytokine production. (a) Representative plots of CD4+ T cells producing IL-4. (b) Total CD4+ T cells producing IL-4, IFN-γ, and IL-17. Results are representative of three independent experiments. *P<0.05; **P<0.01; IFN-γ, interferon-γ; IL, interleukin; NS, not significant; OVA, ovalbumin.
