Figure 2
Airway mucin interactome map: human tracheobronchial epithelial (HBE) secretions were first subjected to S1000 size-exclusion chromatography. Mucins and their interacting proteins were then separated from other structures and protein–protein complexes using isopycnic density-gradient centrifugation. The low-density (T, fractions 1–6), medium-density (M, fractions 8–15), and high-density (B, fractions 16–20) regions were pooled and subjected to a proteomics analysis. Magenta: mucins, Red: proteins found only in the “M” region, Purple: proteins found in both the “M” and “T” regions, Blue: proteins only found only in the “T” region, Green: proteins found in all regions. The size of each proteins cycle reflects its relative abundance. The detected proteins are numbered as follows: 1- LPLUNC1, 2- Ezrin, 3- Actin, 4- Antileukoproteinase 1, 5- Chloride ion channel 1, 6- Complement C3, 7- PLUNC, 8- Dipeptidyl peptidase, 9- Polymeric-IG receptor, 10- Brain acid soluble protein, 11- Tubulin beta-chain, 12- CD9, 13- Gelsolin, 14- Superoxide dismutase, 15- Annexin A2, 16- EHD4, 17- Alpha-enolase,18- Tetraspanin-1, 19- EBP50 20- Complement factor B, 21- Calgizzarin, 22- Peroxiredoxin 1&2, 23- ngALipocalin, 24- Calmyrin, 25- Aldehyde dehydrogenase, 26- Uteroglobin, 27- Pyruvate kinase isozymes M1/M2, 28- MUCIN-1, 29- Glutathione S-transferase P, 30- Cofilin-1, 31- Protein CGI-38, 32- Calcyphosine, 33- Ceruloplasmin, 34- Transgelin-2, 35- Calmodulin, 36- Stomatin, 37- Annexin A5, 38- Calpain-5,6, 39- Prominin 1, 40- Clusterin Apoliporotein J, 41- Cystatin-B, 42- Serpin F1, PEDF, 43- Protein S100 A6,8,9,14,16. 44- DMBT1, 45- Annexin A1, 46- CD59 glycoprotein, 47- Cathepsin D 48- Na/K ATPase alpha-1 chain, 49- Mucin-5B, 50- Prostate stem cell Ag 51- Galectin 9, 52- Leukocyte elastase inhibitor, 53- Galectin-3 BP, 54- Galectin 3, 55- WAP four-disulfide core protein2, 56- Defensin1, 57- Galectin 8, 58- Lysozyme C, 59- Serpins B3&B6 60-MUCIN 4, 61-MUCIN-5AC, 62-MUCIN-16, 63- MUC-20.
