Figure 4
The effect of chaotropic agents on mucin interactome: (a) The effect of GuHCl (0–8 M) on the recovery of the void complexes: the results of the titration experiment show that the first discernible decrease in recovery of the void peak was observed at a GuHCl concentration of approximately 2 M (green; as indicated). The loss increases progressively through 4 M GuHCl (magenta) and 6 M (black) to peak at 8 M (brown). (b) The effect of GuHCl and detergent on the recovery of the void complexes: the panel shows traces of the peak of the V0 region from the S1000 column, as obtained from the Optilab refractometer, and the molar masses, as obtained from Dawn multi-angle laser light scattering (MALLS), plotted across the mucin peak. The starting material, human tracheobronchial epithelial (HBE) cell-culture secretion (diluted in phosphate-buffered saline (PBS)), is denoted by the largest peak (magenta), and the subsequent dilution with 4 M GuHCl (green) and 0.1% CHAPS/0.1% Triton is shown in blue. The results for GuHCl plus the detergent are shown in brown.
