Figure 7
Frequency vs. dissipation changes, ΔD/Δf, during the absorption of mucins, glutathione S-transferase (GST) and galectin-3. The linear ΔD/Δf corresponds to a highly hydrated layer (mucin binding). The addition of albumin changes the ΔD/Δf to a flat or downward slope, suggesting the formation of a rigid layer on the surface. The subsequent GST additions change the ΔD/Δf slope to a linear upward trend, with an increase in the mass bound resulting in greater dissipation (a). The addition of galectin-3 (b) first causes a slight and then sudden decrease in the dissipation and the ΔD/Δf. The trend of the layer hydration/thickness is marked with broken arrows, and the stabilization of the layer at the end of the wash is marked (S).
