Figure 1
Differences in cellular composition of mesenteric lymph node (MLN) and spleen after primary or challenge infection with H. polygyrus bakeri. (a) Adult worm burdens in H. polygyrus bakeri-infected B6 mice on day 14 after primary or challenge infection. Data from individual mice pooled from three independent experiments are shown. Data are presented as mean±s.e.m. ****P<0.0001. (b) Total cell numbers in MLN and spleen on day 7 after primary or challenge infection. *P<0.05; ***P<0.001. (c) Total CD4+GATA3+ T cells in MLN and spleen on day 7 after primary or challenge infection. ns, not significant; *P<0.05; **P<0.01; ***P<0.001 (d and e) CD11b+CD206+ cells were determined by flow cytometry in gated F4/80+ MLN (d) and spleen cells (e) from naive and infected mice on day 7 after primary or challenge infection. The left panel shows representative contour plots from one mouse indicating the frequency of F4/80+CD11b+CD206+, and the right panel shows the absolute cell number. Data are presented as mean±s.e.m. (n=5 mice per group) and are representative of three independent experiments. ns, not significant; **P<0.01; ***P<0.001.
