Figure 2
Primary H. polygyrus bakeri infection induces accumulation of F4/80−CD11bhiGr1hi myeloid-derived suppressor cells (MDSC) that express both Ly6G and Ly6C. (a and b) CD11bhiGr1hi cells were determined by flow cytometry in gated F4/80− cells in mesenteric lymph node (MLN) (a) and spleen cells (b) from naive and infected mice on day 7 after primary or challenge infection. The left panel shows representative contour plots from one mouse indicating the frequency of F4/80−CD11bhiGr1hi cells, and the right panel shows the absolute cell number. Data are presented as mean±s.e.m. (n=5 mice per group) and are representative of three independent experiments. ns, not significant; **P<0.01; ***P<0.001. (c) Cells were gated on the CD206−CD11b+ population and analyzed by flow cytometry for expression of Ly6G and Ly6C. Representative contour plots of MLN and spleen cells from one mouse on day 7 after primary infection are shown (n=5 mice). (d) Accumulation of F4/80+CD11b+CD206+ and F4/80−CD11bhiGr1hi cells in MLN and spleen after primary infection. Data are presented as mean±s.e.m. (n=4 mice per time point). Left panels, F4/80+CD11b+CD206+ cells. Right panels, F4/80−CD11bhiGr1hi cells. ns, not significant; ***P<0.001.
